Binding observing the Ligand Theory: soon.... How to set an STD experiment? 1) Adjust everything at zgesgp (water suppression..); 2) wrpa 2 --> re 2; 3) rpar SCREEN_STD all (stddiffesgp.3); 4) Acqupars --> Lists à FQLIST --> edit à FQ2LIST --> E (edit); … (select); Obs: 1) Reference (ex: -40, 40, 50, or 100 [...]

At higher magnetic fields 15N relaxation is dominated by chemical shift anisotropy (CSA) and Dipole-dipole (DD), which leads to increase of the overall transverse relaxation rates with increasing magnetic field, B0. The TROSY (Transverse Relaxation Optimized SpectroscopY) experiment utilizes the fact that cross-correlated relaxation interference, caused by CSA and DD, are different for the individual multiplet components [...]

Essential points I would like to highlight before the NOESY parameters: Dipole-dipole interaction between two nuclear spins on the same molecule is affected by molecular tumbling, which causes a variation in the magnitude and direction of the magnetic field exerted by one spin to the other. The molecular tumbling causes a fluctuation of the [...]

I like to compare TOCSY with gossip. It does not matter if Hydrogens 1 (H1) and H3 are scalar coupled; if H1 is coupled to H2 and H2 is coupled to H3, we will observe a correlation between H1-H3. However, this transmission is broken if there's an interruption of "communication": J=0 or [...]

Top image: Conventional HSQC Bottom image: CT-HSQC Comparison of the parameters present on the conventional HSQC (hsqcetgpsp) and CT-HSQC (hsqcctetgpsp).   Protein NMR Spectroscopy: Principles and Practice 2nd Edition by Arthur G. Palmer III, Wayne J. Fairbrother, John Cavanagh, Nicholas J. Skelton, Mark Rance.

      Another Option for 15N-HSQC: fhsqcf3gpph. Parameters described below are also valid for fhsqc* below. Fast-HSQC, Phase-sensitive ge-2D 1H-15N HSQC using WATERGATE (3-9-19) (fhsqcf3gpph |  FHSQCF3GPPH) HSQCETF3GPSI2, Phase-sensitive ge-2D 1H-15N HSQC Adjust the Match/Tune for the 15N using atmm – you can do this at the beginning, before running your 1D using edasp. Optional 13C; used [...]

  Create a new dataset (new or edc). Choose pulse program zg30 to record a conventional 1H spectrum. Remember to choose your own folder. Select ZGESGP on the Experiment (Same as mentioned before - ZGPR or PROTON). Type getprosol Check o1p: Set the offset to 4.7 ppm (H2O + D2O) Calibrate the pulse (pulsecal) MODIFY SPECIFIC [...]

   Please, read "BEFORE RUNNING ANY EXPERIMENT" before running ZGPR. Create a new dataset with edc or new. In the Parameters group click Select to open the Experiment Table window (you can just type ZGPR). Select the experiment ZGPR from the table and click on Set selected item in editor. Type “getprosol 1H "pulse length" "power [...]

  Create a new dataset (new or edc). Choose pulse program zg30 to record a conventional 1H spectrum. Choose your own folder. Type rpar PROTON all or Select PROTON. Type getprosol: gets probe and solvent dependent parameters from the edprosol table. Set the offset to 4.7 ppm (H2O + D2O) Calculate the receiver gain by typing rga. [...]

  The Figure below shows, in steps, how to correctly measure your sample using the depth gauge before putting it inside the magnet. Sample Volume: 5 mm tube: 500-600 µL 3 mm tube: 180-200 µL 1.7 mm tube: 50-60 µL Solids and bubbles are NOT welcome. Insert the sample [if using Sample Case (ASRC: [...]