15N – HSQC

Another Option for ¹⁵N-HSQC: fhsqcf3gpph. Parameters described below are also valid for fhsqc* below.

• Fast-HSQC, Phase-sensitive ge-2D ¹H-¹⁵N HSQC using WATERGATE (3-9-19) (fhsqcf3gpph |  FHSQCF3GPPH)
• HSQCETF3GPSI2, Phase-sensitive ge-2D ¹H-¹⁵N HSQC
• Adjust the Match/Tune for the ¹⁵N using atmm – you can do this at the beginning, before running your 1D using edasp.
• Optional ¹³C; used for decoupling ¹³C during t1 in doubly-labeled proteins: type eda and on zgoptns type “-DLABEL_CN”. 
• Obs: f stands for fast.

D19 is a small number 62.4 µs (800 MHz). On the pulse sequence, ; so in this case  d = 4006 Hz or 5 ppm. An even better number on this case would be d19= 44 µs – d = 7 ppm (14/2).

If you’re uncertain, 100 µs is a good number.

Fundamentals of Protein NMR Spectroscopy (Focus on Structural Biology, 5) 2006th Edition by Gordon S. Rule, T. Kevin Hitchens.